Xenbase Image ID: 83823

Fig. 2. LR is expressed in animal intestine throughout development. A: Northern blot analysis reveals that LR mRNA is expressed through intestinal development while ST3 expression is limited to the intestinal metamorphosis period. Total RNA was isolated from intestine of tadpoles at various stages during metamorphosis. Ten micrograms of each were electrophoresed on an agarose gel and hybridized with ST3 and LR probes. Note that ST3 mRNA shows dramatic upregulation during the early stages of metamorphic climax (stages 60, 61) whereas little change was found in LR mRNA level during metamorphosis. B: Western blot shows that LR protein is also present at all stages of intestinal metamorphosis. Total protein was isolated from intestine of tadpole at various stages during metamorphosis. One microgram of each was electrophoresed on an SDS gel and detected by anti-Xenopus LR antibody. The open arrow indicates full-length LR. The triangle and solid and open circles indicate possible degradation products (as described later). Note that the bands indicated by circles were present only at stage 61, the climax of intestinal remodeling, while the largest degradation product (triangle) was observed in different sample preparations at different stages (data not shown) and did not correlate with intestinal metamorphosis. Image published in: Amano T et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

Return to previous page