XB-IMG-83831
Xenbase Image ID: 83831
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Fig. 5. Misexpression of XSmo and mHIP alters the expression of the placodal marker genes XEya1 and XPax-8. Two-cell-stage embryos were injected into
one blastomere with XSmo (A, C, D) mRNA (1.5 ng) or mHIP (B, E-G) mRNA (B, 750 pg; E-G, 500 pg), fixed at early neurula stages (13-14) and analyzed
for XEya1 or XPax-8 expression (purple). Embryos are shown in dorsal view in (A) and (C-E) (anterior is down), anterior view in (B) and lateral view in
(F) and (G) [anterior is right (F) or left (G)]. Embryos in which XEya1 or XPax-8 signals overlapped with -galactosidase-positive regions (light blue) were
analyzed. Manipulated embryos exhibited enhanced and expanded XEya1 expression in the preplacodal region (XSmo: 59%, n 22; mHIP: 60%, n 25).
Posterior expansion and enlargement of the XPax-8-positive preotic domain (located anteriorly) was observed in 57% (n 28) and 53% of XSmo-injected
embryos, respectively (C), with a concomitant reduction of pronephric XPax-8 expression (C, 46%, n 28). The pronephric and the preotic domain merged
in 18% (n 28) of XSmo-injected embryos (D) and in 60% (n 10) of mHIP-injected embryos (E, F). In a subset of mHIP-injected embryos, an expansion
of the preotic domain (40%, n 10) and a reduction of the pronephric domain (20%, n 10) were also observed (not shown). Representative examples
are shown for each injected construct. is, injected side; nis, noninjected side. Image published in: Koebernick K et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V.
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