Xenbase Image ID: 83893

Fig. 2. Temporal and spatial expression of Nbx during Xenopus development. (A) Temporal expression patterns of Nbx. RNA was extracted from embryos at the indicated stage of development. Nbx mRNA expression levels were measured by RT-PCR. The ubiquitous marker histone H4 served as a control. (B�I) Spatial expression patterns of Nbx. Nbx expression was analyzed by whole-mount in situ hybridization. (B) Stage 12. Black arrowheads in (B) indicate outer stripes, and white arrowheads indicate inner stripes of Nbx expression. (C) Stage 14. (D) Stage 18. (E) Stage 20. (F) Stage 25. (G) Stage 30. Arrow in (G) indicates new expression of Nbx in mesencephalon, and a transverse section of this region is shown in (I). (H) A transverse section showing the outer stripes at stage 14. (I) A transverse section of Nbx-expressing region in mesencephalon at Stage 30. (J�T) Comparison between recognized markers and Nbx expression. Whole-mount double in situ hybridizations were performed with a digoxigenin-labeled Nbx probe and a fluorescein-labeled probe for another marker. (J) Nbx (purple) and Otx2 (blue) at stage 14. (K) Nbx (purple) and Sox2 (blue) at stage 14. (L) Nbx (purple) and N-CAM (blue) at stage 16. (M, N) Nbx (purple) and Slug (blue) at stage 16. The 1 in (M) indicates the region where only Slug was expressed; the 2 indicates where Slug and Nbx were coexpressed (a transverse section of this region is shown in N); and the 3 indicates where only Nbx was expressed. (O) Nbx (purple) and Delta-1 (blue) at stage 16. (P) Nbx (purple) and -tubulin (blue) at stage 16. (Q) A schematic representation of the expression of Nbx and -tubulin. (R) An enlarged view of the outer stripe region at stage 16. Nbx (purple) and -tubulin (blue). (S, T) Nbx (purple) and - tubulin at stage 25. The transverse section of posterior region is shown in (T). Image published in: Kurata T and Ueno N (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue nkx1-2.L Nbx X. laevis Throughout NF stage 12 ectoderm nkx1-2.L Nbx X. laevis Throughout NF stage 14 neural crest cranial neural crest neural plate chordal neural plate neural plate border nkx1-2.L Nbx X. laevis Throughout NF stage 18 neural plate chordal neural plate neural fold neural plate border chordal neural plate border cranial neural crest hyoid crest branchial crest nkx1-2.L Nbx X. laevis Throughout NF stage 20 neural tube posterior neural tube cranial neural crest nkx1-2.L Nbx X. laevis Throughout NF stage 25 neural tube posterior neural tube posterior nkx1-2.L Nbx X. laevis Throughout NF stage 29 and 30 midbrain spinal cord posterior nkx1-2.L Nbx X. laevis Throughout NF stage 16 neural plate border chordal neural plate border cranial neural crest hyoid crest branchial crest otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 14 anterior pre-chordal neural plate sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 14 neural plate ncam1.S n-cam, n-cam-1, ncam, ncam1-a, ncam1-b X. laevis Throughout NF stage 16 neuroectoderm snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 16 cranial neural crest mandibular crest hyoid crest branchial crest dll1.L delta-1, Delta-1, delta1, x-delta, X-delta-1, Xdelta-1, Xdelta1, XDelta1 X. laevis Throughout NF stage 16 neural plate tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 16 neuron tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 25 central nervous system spinal cord neuron Image source: Published Larger Image Printer Friendly View

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