XB-IMG-83894
Xenbase Image ID: 83894
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Fig. 3. Nbx expression coincide with neural and neural crest formation. We injected the mRNAs to several genes into embryos and examined the effect of
their forced overexpression on Nbx expression by whole-mount in situ hybridization. (A) BMP4 (200 pg) reduced Nbx expression. (B) Overexpression of
tBR (500 pg) by injection into the ventral region expanded Nbx expression into the induced secondary axis. (C) Xngnr1 (100 pg) reduced Nbx expression.
(D) Xngnr1 (100 pg) induced ectopic -tubulin expression. (E, F) Xotch E (200 pg) injected embryos. (E) Xotch E reduced Nbx expression. (F) Xotch E
reduced -tubulin expression. (G) Zic3 (200 pg) caused ectopic Nbx expression (black arrowheads). The ectopic expression was limited to the posterior region
of the injected embryos (white arrowhead). (H) Zic3 (200 pg) caused ectopic Slug expression. (I) Zic5-ZFC (200 pg) reduced Nbx expression. (J) Zic5-ZFC
(200 pg) reduced Slug expression. (K) Zic5-ZFC (200 pg) reduced Sox2 expression. Image published in: Kurata T and Ueno N (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V.
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