Xenbase Image ID: 83895

Fig. 4. Nbx acts as a transcriptional suppressor. (A) Schematic representation of Nbx, VP-Nbx, and EnR-Nbx constructs. (B) Phenotypes of Nbx (200 pg)-injected embryos. The Nbx injection into the dorsal side caused a head defect. (C) Uninjected embryos developed normally. (D, E) Wholemount in situ hybridization analysis of Nbx (200 pg)- and -gal (100 pg)-injected embryos at stage 11. Nbx suppressed the organizer markers Otx2 (D) and goosecoid (E). (F, G) Whole-mount in situ hybridization analysis of -gal (100 pg)-injected embryos. -gal injection did not affect the expression of Otx2 (F), goosecoid (G). (H) Phenotype of VP-Nbx (100 pg)-injected embryo. The VP-Nbx injection into the ventral side caused ectopic cement gland formation (arrowhead in H). (I:) Uninjected embryos developed normally. (J�M) Whole-mount in situ hybridization analysis of VP-Nbx (100 pg)- and -gal (100 pg)-injected embryos. VP-Nbx injection into the dorsal side led to the expanded expression of Otx2 (J) and goosecoid (K). VP-Nbx injection into the ventral side resulted in the ectopic expression of Otx2 (L) and goosecoid (M) at the injected region. (N) Phenotype of EnR-Nbx (100 pg)-injected embryo. The injection caused a head defect. (O:) Uninjected embryos developed normally. Image published in: Kurata T and Ueno N (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 11 dorso-lateral marginal zone gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 11 upper blastopore lip Image source: Published Larger Image Printer Friendly View

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