Xenbase Image ID: 83896

Fig. 5. Overexpression of Nbx in lateral neural plate suppressed neural plate makers and caused enhanced neural crest formation. (A) Schematic representation in the Nbx construct. (B�I) Whole-mount in situ hybridization analysis of Nbx-injected embryos. Each panel shows two embryos with the same treatment. (B�D) Expression of neural plate makers. Overexpression of Nbx suppressed neural plate makers Otx2 (B), N-CAM (C), and Sox2 (D). (E) Overexpression of Nbx expanded the expression of epidermal keratin into the neural plate. (F�I) Expression of neural crest maker Slug. Slug was reduced at stage 15 (F), but enhanced Slug expression was observed at injected region at stage 20 (G, I) and stage 25 (H), (J) RT-PCR analysis of gene expression in the animal caps at stage 20 equivalent that were injected with 300 pg tBR mRNA (lane 1), coinjected with 300 pg tBR and 100 pg Nbx mRNA (lane 2), uninjected (lane 3), and injected with 100 pg Nbx mRNA alone (lane 6). The expression of makers in sibling whole embryo (lane 4). The control reactions with no reverse transcription step (lanes 5 and 7). (K) Animal caps coinjected with 300 pg tBR and 100 pg Nbx contained melanophores at stage 40 equivalent (Black spots in K). (Blown pigments were not melanophores, they were pigment granules derived from the egg.) (L) Uninjected animal caps at stage 40 equivalent. Image published in: Kurata T and Ueno N (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 16 pre-chordal neural plate ncam1.S n-cam, n-cam-1, ncam, ncam1-a, ncam1-b X. laevis Throughout NF stage 16 neural plate sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 16 neural plate krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Throughout NF stage 16 epidermis ectoderm non-neural ectoderm snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Sometime during NF stage 15 to NF stage 20 neural crest cranial neural crest Image source: Published Larger Image Printer Friendly View

Return to previous page