XB-IMG-83929
Xenbase Image ID: 83929
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Fig. 1. Par3 is required for NC migration in Xenopus. (A) Western analysis of Par3 using protein extracts from Xenopus embryos injected with ControlMO or Par3MO. Band intensity is shown relative to ControlMO and normalised to the loading control (MAPK). Arrows indicate individual Par3 and MAPK bands. Error bars show s.d. The experiment was repeated three times; the difference between control and Par3MO was significant (P<0.005). (B) Dorsal view of stage 16 Xenopus embryo injected unilaterally with Par3MO (asterisk) and processed for in situ hybridisation against Snail2, FoxD3, Sox9 and Sox10. (C) Par3MO does not affect NC induction (n=181). n.s., not significant. (D-F) Lateral view of Xenopus embryos showing Twist expression for ControlMO (D), Par3MO (E) or Par3MO co-injected with mRNA for zebrafish Par3GFP (F). Asterisk marks the eye. (G) Percentage of embryos with normal NC migration. Par3MO, n=45; ControlMO, n=24; P<0.001; Par3MO+Par3GFP, n=34; P<0.001. (H) Distance migrated by NC cells relative to mean migration in control embryos. Migration is reduced in Par3MO-injected embryos (P<0.001) but co-injection of Par3GFP with Par3MO rescues migration (P<0.001). (I,J) Single frames from time-lapse movies showing control (I) and Par3MO-injected (J) explants and Delaunay triangulation at 0 and 8 hours. Scale bar: 100 μm. (K) Dispersion between cells increases over time in control but not in Par3MO-injected explants (n=8 explants for each condition, more than 30 cells analysed per explant; *P<0.05, **P<0.01, ***P<0.001). (L) Speed of single NC cells. n=20 per experiment, from three independent experiments; P=0.2146. (M) Persistence of single NC cells. n=20 experiment, from three independent experiments; P=0.4021. (K-M) Error bars indicate s.d. Image published in: Moore R et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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