Xenbase Image ID: 83935

Fig. 4. Par3 is required for CIL in vitro in Xenopus. (A-D) Explant confrontation assay. (A-C) Single frames from time-lapse movies showing maximum overlap between two control explants (A), two Par3MO-injected explants (B) or one control and one Par3MO-injected explant (C). (D) Overlap area as a percentage of total explant area. Control/control, n=27; Par3MO/Par3MO, n=16; P=0.0026; control/Par3MO, n=9; P=0.0252. (E-G) Protrusions overlap analysis for ControlMO-injected (E) or Par3MO-injected (F) cells expressing mbGFP. Magenta overlay shows the protrusion overlap. (G) Protrusion overlap area as a percentage of cell area. Par3MO, n=40; control, n=50; P=0.0002. (H-J) Cell collision assay. (H) Schematic representation of cell collision. Cell position was analysed at 15-minute intervals to measure velocity following collision between ControlMO-injected (I) or Par3MO-injected (J) cells. Velocity vectors were clustered away from the contact in control cells (n=10; P<0.001) but a change in velocity following collision was not observed in Par3MO-injected cells (n=10; P<0.001). Red arrows indicate the initial velocity vector. *P<0.05, **P<0.01, ***P<0.001. Error bars indicate s.d. Scale bars: 100 μm in A; 10 μm in E. Image published in: Moore R et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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