Xenbase Image ID: 83941

Fig. 7. Par3 affects microtubule dynamics at the cell-cell contact in Xenopus. (A-C) EB3GFP-labelled cells were used to analyse microtubule catastrophe. (A,B) ControlMO-injected (A) and Par3MO-injected (B) cells undergoing collision; consecutive frames are presented from time-lapse movies showing one microtubule (time is given in seconds). The boxed area is shown at higher magnification at the cell-cell contact. Arrowhead indicates the highlighted microtubule. Arrow indicates microtubule in contact with the membrane. Circle indicates site of microtubule collapse. (C) Summary of microtubule collapse at the free edge or cell contact. Control cells: n=150 from six independent experiments; *P=0.0167. Par3MO-injected cells: n=100, from six independent experiments; P=0.3. (D-K) plusTipTracker analysis of microtubules at cell-cell contacts. (D-F) Microtubule tracks in ControlMO-injected (D) and Par3MO-injected (E) cells were separated into four groups based on growth speed (slow <26 μm/minute 0.05), depolymerisation rate (I; P>0.05), rescue rate (J; P>0.05) or pause duration (K; P>0.05). However, Par3MO-injected cells exhibited a lower catastrophe rate at the contact compared with control cells (G; *P=0.035). A minimum of ten cells and 25 microtubules per cell were analysed from three independent experiments. Error bars indicate s.d. Image published in: Moore R et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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