XB-IMG-83943
Xenbase Image ID: 83943
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Fig. 8. The Par3 morphant phenotype is rescued with nocodazole or NSC23766 in Xenopus. (A) Dispersion between cells over time under the following treatments: control, Par3MO, Par3MO/nocodazole, Par3MO/NSC23766. n=10 explants for each condition, more than 30 cells analysed per explant; *P<0.05, **P<0.01, ***P<0.001. (B) Speed of cell migration. Control, n=110; Par3MO, n=120; P=0.0503; Par3MO/nocodazole, n=70; ***P=3.751x10-11; Par3MP/NSC23766, n=50; P=0.0660. (C) Persistence. Control, n=100; Par3MO, n=120; P=0.3131; Par3MO/NSC23766, n=50; P=0.7696; Par3MO/nocodazole, n=70; **P=0.0064. (D-H) Explant confrontation assay. Single frames from time-lapse movies showing maximum overlap between control explants (D), Par3MO-injected explants (E), Par3MO-injected explants in nocodazole (F) and Par3MO-injected explants in NSC23766 (G). Scale bar: 100 μm. (H) Overlap area as a percentage of total explant area (n=23; *P<0.05). (I-L) Cell collision assay. Cell positions were analysed at 15-minute intervals to measure the velocity following collision. Velocity change was clustered in control cells (I; n=10; P<0.001) but no velocity change was observed in Par3MO-injected cells (J; n=10; P<0.005). However, velocity vectors were clustered away from the cell contact in Par3MO-injected cells in nocodazole (K; n=10; P<0.001) and Par3MO-injected cells in NCS23766 (L; n=10; P<0.001). Red arrows indicate the initial velocity vector. Image published in: Moore R et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |