XB-IMG-83948
Xenbase Image ID: 83948
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Fig. 10. TrioMO rescues the Par3MO-induced decrease in microtubule catastrophe and increase in Rac activity at contacts in Xenopus. (A-D) EB3GFP-labelled cells were manually analysed for microtubule catastrophe. (A-C) ControlMO-injected (A), Par3MO-injected (B) and Par3MO+TrioMO-injected (C) cells undergoing collision; consecutive single frames are presented from time-lapse movies showing one microtubule. The boxed area is shown at higher magnification at the cell-cell contact. Arrowhead indicates the highlighted microtubule. Arrow indicates microtubule in contact with the membrane. Circle indicates site of microtubule collapse. (D) Microtubule catastrophe. Controls versus Par3MO, n=5, *P=0.0258; control versus Par3MO+TrioMO, n=5, P=0.3070. (E) Analysis of EB3GFP-labelled cells using plusTipTracker. *P<0.05. (F,G) FRET analysis of Rac activity in NC. (F) Rac FRET efficiency for control, Par3MO- and Par3MO/TrioMO-injected cells. (G) Rac FRET efficiency at the cell contact. Fifteen cells were analysed per condition from a minimum of four individual explants. *P<0.05. Error bars indicate s.d. Image published in: Moore R et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |