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Fig. 5. The Effects of Oct25 on convergence and extension movements analyzed with different assays. (A) Shape changes of explants of the dorsal marginal zone (DMZ) were measured by calculating the quotient of length and width (l/w). Explants were excised with l/w % 1. After cultivation for 24 h, explants were classified as shown. l/w 1.5 is defined as "not elongated", 1.5 < l/w < 2.5 is defined as "weak elongation", l/w > 2.5 is defined as "strong elongation". (B) Changed elongation after injection of Oct25 mRNA in the animal pole (Oct25 in animal cap [AC]), in the marginal zone (Oct25 in MZ), and in the vegetal pole (Oct25 in VG). In addition, the effect on Acti- vin A induced elongation is shown by comparing Activin A induced ACs with (Oct25 in AC+act.) and without Oct25 injection (uninj. AC +act.). Asterisks indicate high significance (v2 test, a 0.01). (C) AC explants (AC) without treatment (left), AC induced with Activin A (middle), AC injected with Oct25 mRNA and treated with Activin A (right). (D) Photographs of embryos labeled with red EosFP in the DMZ. The anterior-posterior axis is diagonal, the blastopore to the lower left corner (dashed line). Left side: Uninjected embryo at begin- ning of gastrulation and at the end. Right side: Embryo injected with Oct25 mRNA at the corresponding stages. Note that the blastopore remains open in the injected embryos. Corresponding values are shown in Table S5. Image published in: Julier A et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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