Xenbase Image ID: 84040

Fig. 1. XDSCR6 triggers the formation of dorsal mesoderm and neural tissues in Xenopus ectoderm explants. (A,B) Morphology of control (A) and Xdscr6-injected (B) explants at the early neurula stage equivalent. (C-H) In situ hybridisation analyses of the expression of MLC, XCG1 and Sox3 genes in control (C,E,G) and Xdscr6-injected (D,F,H) ectoderm explants at the late neurula stage equivalent. (I) RT-PCR analyses of gene expression in ectoderm explants cultured to stage 10.5 and 20 equivalent, following injection of the indicated amounts of Xdscr6 mRNA, Xdscr6 DNA or after activin treatment. ODC was used as a loading control. Ms., muscle specific. (J) Real-time RT-PCR analyses of the expression level of the indicated genes in early gastrula ectoderm explants injected with low and high amounts of Xdscr6 mRNA (top row) or treated with low and high doses of activin (bottom row). Similar results were obtained using ODC or EF1α as reference. Image published in: Li HY et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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