Xenbase Image ID: 84041

Fig. 2. XDSCR6 induces a secondary embryonic axis. (A) Xdscr6 mRNA or DNA was injected into one ventral blastomere (arrow) at the 4-cell stage. (B,C) Vegetal view of an uninjected early gastrula embryo (B) and a Xdscr6 mRNA-injected early gastrula embryo showing the precocious formation of the ventral blastopore (C, arrow). (D,E) Dorsal view, with anterior region at the top, of an uninjected late neurula embryo (D) and a Xdscr6 mRNA-injected late neurula embryo with a secondary neural tube (E). (F-H) Phenotypes of control (F), Xdscr6 mRNA-injected (G) and Xdscr6 DNA-injected (H) embryos at stage 35. (I,J) β-galactosidase staining of stage 32 embryos injected with lacZ mRNA alone (I) or co-injected with Xdscr6 mRNA (J). (K) Transverse section corresponding to J showing β-galactosidase staining in XDSCR6-induced muscles. (L-O) In situ hybridisation analyses of the expression of the indicated genes in Xdscr6 mRNA-injected embryos at stage 32. I and II designate the primary axis and the secondary axis, respectively. Image published in: Li HY et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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