Xenbase Image ID: 84042

Fig. 3. Dorsalisation of ventral mesoderm by XDSCR6. (A-H) In situ hybridisation analyses of Otx2, chordin, Wnt8 and Xvent1 expression in uninjected (A,C,E,G) and Xdscr6-injected (B,D,F,H) embryos at the early gastrula stage. Ectopic Otx2 and chordin expression sites are indicated by arrowheads. (I) Scheme of the dorsalisation experiment. Embryos were ventrally injected at the 4-cell stage (double arrows) and DMZ (D) or VMZ (V) explants were dissected at the early gastrula stage and cultured to late neurula stage equivalent. (J-M) Morphology of the uninjected and injected DMZ or VMZ explants. (N) RT-PCR analyses of gene expression in uninjected and injected DMZ or VMZ explants. (O) In situ hybridisation of MLC and XCG1 expression in ectoderm explants injected with the mRNAs indicated at the top and cultured to late neurula stage equivalent. DMZ, dorsal marginal zone; VMZ, ventral marginal zone. Image published in: Li HY et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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