XB-IMG-84043
Xenbase Image ID: 84043
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Fig. 4. XDSCR6 is required for anterior development. (A,B) Dorso-vegetal view of chordin expression in a CoMO (A) and a Xdscr6MO-injected (B) embryo at stage 11. (C,D) Dorso-vegetal view of noggin expression in a CoMO (C) and a Xdscr6MO-injected (D) embryo at stage 10.5. (E,F) Dorso-vegetal view of goosecoid expression in a CoMO (E) and a Xdscr6MO-injected (F) embryo at stage 10. (G,H) Anterior view of Otx2 expression in a CoMo (G) and a Xdscr6MO-injected (H) embryo at stage 20. (I,J) Ventral view, with anterior region at the top, of XCG1 expression in a CoMO (I) and a Xdscr6MO-injected (J) embryo at stage 20. (K,L) Dorsal view, with anterior region at the top, of Sox3 expression in a CoMO (K) and a Xdscr6MO-injected (L) embryo at stage 18. (M-O) Phenotypes of a CoMo-injected embryo (M), a Xdscr6 morphant embryo (N) and a myc-Xdscr6 rescued embryo (O) at stage 38. (P) Summary of the rescue results. Numbers at the top indicate total embryos scored from four independent experiments. (Q) Western blot analysis shows the blockade of protein synthesis from Xdscr6-Flag mRNA, but not from myc-Xdscr6 mRNA, by Xdscr6MO. Tubulin is a loading control. CoMO, mismatch control MO. Image published in: Li HY et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |