Xenbase Image ID: 84044

Fig. 5. The WRPW motif is necessary and sufficient to induce a secondary axis. (A) Structure of the various Xdscr6 constructs. (B) Summary of secondary axis formation in control (Ctl) and injected embryos (constructs numbered as in A). XDSCR6 (1), XDSCR6N (2), XDSCR6-EnR (5), XDSCR6-VP16 (6), myc-WRPW (7) and GFP-WRPW (9) similarly induce a secondary axis, whereas XDSCR6C (3), XDSCR6m (4), myc-WRSG (8) and GFP-WRSG (10) have no effect. The results are expressed as a percentage and numbers at the top indicate total embryos scored from three independent experiments. (C) A GFP-WRPW-injected stage 35 embryo with green fluorescence concentrated in the anterior region of the secondary axis (arrow). (D) A GFP-WRSG-injected embryo lacks a secondary axis, with green fluorescence distributed in the posterior region. (E) In situ hybridisation analyses of chordin expression at stage 10.5 following overexpression of XDSCR6-EnR, XDSCR6-VP16 or myc-WRPW in the ventro-vegetal region at the 4-cell stage. All embryos are in vegetal view. Image published in: Li HY et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page