Xenbase Image ID: 84046

Fig. 7. XDSCR6 interferes with the nuclear localisation of endogenous Ezh2 and BMI1. Confocal microscopy analyses of Ezh2- and BMI1-associated PcG bodies in HeLa cells. In cells transfected with Xdscr6-RFP or myc-WRPW (arrowheads in A-C,M-O,I-K,U-W), the intensity of endogenous Ezh2 and BMI1 staining is significantly decreased compared with neighbouring untransfected cells in the same culture dish (arrows). In Xdscr6m-RFP-transfected cells (arrowheads in E-G,Q-S), the intensity of fluorescence is comparable to that of neighbouring untransfected cells (arrows). Scale bar: 10 μm. (D,H,L,P,T,X) Quantification of nuclear fluorescence intensity. Red lines represent the median for each condition. Data are presented as mean ± s.e.m. P-values (Student’s t-test) and number of measures (n) are indicated. Image published in: Li HY et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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