Xenbase Image ID: 84100

Fig. 7. Elevated retinoid signalling in the ectodermal component of neural-inducing conjugates results in the increased expression of the primary neuron marker, NST. (A–C) NST expression in conjugates of animal cap ectoderm and dorsolateral mesoderm, grown in 10−7 M RA and assayed at the late neurula stage for the expression of NST. The component injected with RAR/RXR mRNA is indicated in green print. Expression of NST is increased (black arrows) in area in conjugates that received RAR/RXR in the animal cap component (C) compared to controls (A). Conjugates with additional RAR/RXR transcripts in the mesoderm (B) showed less and reduced NST expression possibly because the retinoid signalling ventralizes the mesoderm (Ruiz i Altaba and Jessell, 1991) and makes it a poorer neural inducer. (D,E). A section through one of the conjugates shown in (C) seen under normal (D) and uv (E) light to correlate NST expression (blue patches indicated by arrows) in (D) with the animal cap ectodermal component of the conjugate (green fluorescence) in (E). Image published in: Sharpe C and Goldstone K (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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