XB-IMG-84156
Xenbase Image ID: 84156
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Figure 3. miR-142-3p Is Required for Definitive Hemangioblast Specification and Is Sufficient to Rescue Hemangioblast Programming in dgcr8 Morphants
(A–C) LOF experiment: miR-142-3p is required for formation of hemogenic endothelium and for hemangioblast specification in the DLP. Embryos injected with 100 ng miR-142-3p MO (A), but not a mismatch control MO (B), phenocopy hematopoietic defects of Dgcr8 MO embryos. Top: WISH showing runx1 expression in hemogenic endothelium (arrowheads) at stage 39. Bottom: WISH showing flk1 expression in DLP hemangioblasts (red arrows) at stage 24. Endothelial cells in VBI are indicated by black arrows. (C) WISH showing flk1 expression is normal in the DLP (arrows) at stage 24 in miR-142-5p (passenger strand) morphants.
(D) Rescue experiment: Coinjection of the miR-142-3p mimic is sufficient to restore hemangioblast formation in dgcr8 morphants. Expression of flk1 (top) and scl (bottom) in the DLP (arrows) analyzed by WISH at stages 24–25.
(E) GOF experiment: Enhanced hematopoietic programming by overexpression of miR-142-3p. Left: WISH of runx1 at stage 39 shows that the hemogenic endothelium (arrowheads) is expanded posteriorly out of the trunk, into the postcloacal tail region of the DA (arrows) in the miR-142-3p mimic-injected embryos. Dashed line indicates the location of the cloaca. Right: WISH analysis of flk1 expression in the DLP hemangioblasts (arrows) at stages 24–25. The number of embryos represented by each panel, out of the number analyzed, is indicated in the top right corner. The anterior of the embryo is to the left and dorsal to the top in all images. See also Figure S2. Image published in: Nimmo R et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |