Xenbase Image ID: 84158

Figure 5. Vasculogenesis Defects in miR-142-3p Morphants (A) Hemogenic endothelium is not formed in miR-142-3p morphants. In situ hybridization analysis of gfi1a and spib expression in the midline (arrowheads) at stage 39. (B) Endothelial differentiation and migration defects in miR-142-3p morphants. WISH analysis of flt4, flk1, and flt1 expression in DLP-derived angioblasts in the PCV (arrows) and in cells migrating to the midline to form the DA (arrowheads) at stage 30. (C) Defective expression of endothelial markers in miR-142-3p morphants. WISH analysis of AA4.1, CD31, dll4, and cx37 in the DA (arrowhead) and PCV (arrows) at stage 34. (D) A residual DA is formed in miR-142-3p morphants (as shown by WISH analysis of CD31, arrowhead) but fails to switch on the hematopoietic TFs gata2 at stage 34 or runx1 at stage 36, as shown by WISH. Position of the DA is indicated by arrowheads. The number of embryos represented by each panel, out of the number analyzed, is indicated in the top right corner. The anterior of the embryo is to the left and dorsal to the top in all images. See also Figure S4. Image published in: Nimmo R et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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