Xenbase Image ID: 84173

Figure 2. Misexpression of mZac1c in Xenopus retina biases progenitors to acquire retinal ganglion cell (RGC) and M�ller glial cell fates. A: Schematic illustration of a mature stage 40 Xenopus retina. B: Stage 40 control-injected retina showing green fluorescent protein (GFP) epifluorescence (green) of transfected cells. C�F: Stage 40 retina co-injected with expression constructs for GFP and myc-tagged Zac1, showing GFP epifluorescence (green, C,E,F) and immunostaining with anti-myc (red, D,E). F is a high-magnification image of the boxed area in C, showing a representative abnormal cluster of RGC layer (GCL) cells induced by Zac1. G,H: Anti-Zac1 immunostaining (red, G,H) of stage 40 Zac1+GFP (green, H) injected retina. Note that Zac1 antibody does not cross-react with Xenopus Zac1. I: Quantification of GFP+ cells corresponding to each retinal cell class, showing a significant (***P < 0.001) expansion of M�ller glial and cells in the RGC layer at the expense of most other cell types after misexpression of mZac1c. n = number of retinae analyzed, and total cell counts were 1,553 for control and 1,146 for mZac1c. Error bars are SEM. gcl, ganglion cell layer; inl, inner nuclear layer; onl, outer nuclear layer; le, lens; PR, photoreceptors; Am, amacrine cells; Bp, bipolar cells; Mu, M�ller glia; Hc, horizontal cells; RGC, retinal ganglion cells. Image published in: Ma L et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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