Xenbase Image ID: 84403

Fig. 2. Do the VegT and Wnt pathways act separately in the vegetal mass to produce a mesodermal signal and a dorsal signal respectively? (A) The design of the experiment shown in C. The equatorial regions of β-catenin– embryos at stage 8 were dissected and co-cultured for 2 hours with wild-type, β-catenin– or VegT– vegetal masses. The explants were then separated and cultured until siblings reached the mid-gastrula stage, for analysis of dorsal mesodermal markers, and the mid-neurula stage, for the examination of convergence extension movement and the late mesodermal marker, MyoD. (B) The appearance of an equatorial region/vegetal mass co-culture. (C) The appearance of the equatorial regions after separation from the vegetal masses and overnight culture until siblings reached neurula stage 16. (D) Real-time RT-PCR to show the relative levels of expression of organizer genes in sibling equators of those shown in C and D, frozen at the gastrula stage (11, or 16 in last panel). EQ, equator; β-cat–/wt bs, β-catenin– equator co-cultured with wild-type bases; β-cat–/VegT– bs, β-catenin– equator co-cultured with VegT– bases. In each case, ornithine decarboxylase (ODC) is used as a loading control (data not shown), and each bar is normalized to the level of ODC. Image published in: Xanthos JB et al. (2002) Copyright © 2002. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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