Xenbase Image ID: 84405

Fig. 4. The timing, extent and localization of expression of Xnrs is regulated by the Wnt pathway and is necessary for inducing dorsal mesoderm in equatorial cells. (A) Real-time RT-PCR to show the relative levels of expression of the nodal genes Xnr1, 2, 3 and 4 in dorsal and ventral halves of wild-type and β-catenin– embryos frozen at the late blastula and early gastrula stages (9, 9.5 and 10). The dorso-ventral pattern of expression of Xnr1 seen in wild-type halves, is lost in β-catenin– embryos. ODC is used as a loading control (data not shown), and each bar is normalized to the level of ODC. (B) The design of the experiment shown in C. Equators from wild-type and β-catenin– mid-blastula stage embryos were co-cultured with with β-catenin– vegetal masses from mid-blastulae (β-cat–/β-cat– early) or early gastrula stage (β-cat–/β-cat– late). Controls were mid blastula β-catenin– equators co-cultured with wild-type mid-blastula vegetal masses (β-cat–/WT). The co-cultures were then separated and equators were cultured until siblings reached the late neurula stage and they were then frozen for analysis. (C) Real-time RT-PCR to show the relative levels of expression of MyoD in equators from the experiment described in B. ODC is used as a loading control (data not shown), and each bar is normalized to the level of ODC. Image published in: Xanthos JB et al. (2002) Copyright © 2002. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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