Xenbase Image ID: 84407

Fig. 6. The importance of a Smad1 phosphorylation- free zone in axis formation. (A) Embryos depleted of maternal (m βcat–), or both maternal and zygotic (m/z βcat–) β-catenin were injected with cm-BMP7 mRNA (600 pg into 1 ventral vegetal cell at the 8-cell stage) and photographed at the tailbud stage. c, cement gland; nt, neural tube. (B) Embryos depleted of maternal VegT mRNA were injected with cm-BMP7 mRNA (600 pg into 1 ventral vegetal cell at the 8-cell stage and photographed at the tailbud stage. c, cement gland. (C,D) cm-BMP7 mRNA (600 pg) together with lacZ mRNA (200 pg) was injected into one ventral cell at the 8-cell stage, in both m β-catenin– and m/z β-catenin– embryos, that were then photographed at the tailbud stage. Blue cells derived from the injected blastomere were visible in cleared embryos mostly in the rescued notochord and neural structures. b, brain; n, notochord nt, neural tube. (E) Histological section of an m/z β-catenin– embryo rescued by cm-BMP7/Xga mRNA injection. Note the blue cells in notochord (n) and neural tube (nt) in this phase contrast picture. (F) Hematoxylin and Eosin stained histological sections of wild-type, m/z β-catenin– and m/z β-catenin– +cm-BMP7 mRNA injected embryos at the late tailbud stage. n,notochord; nt,neural tube. Image published in: Xanthos JB et al. (2002) Copyright © 2002. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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