XB-IMG-84408
Xenbase Image ID: 84408
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Fig. 7. Organizer gene expression is rescued at the early neurula stage by BMP blockade in β-catenin– but not VegT– embryos. Wild-type (uninjected, U), VegT– thioate (V), m β-catenin– thioate (T) and m/z β-catenin– morpholino (M) embryos, and siblings injected with cm-BMP7 (cmB) at the 8-cell stage (600 pg; 1 ventral vegetal cell) were frozen at the early gastrula (stage 10.5) and early neurula stage (stage 13) and analysed by real-time RT-PCR for organizer gene expression. The results are displayed as histograms to show the relative levels of expression. In each case, ornithine decarboxylase (ODC) is used as a loading control (data not shown), and each bar is normalized to the level of ODC expression. Image published in: Xanthos JB et al. (2002) Copyright © 2002. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |