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XB-IMG-84408

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Fig. 7. Organizer gene expression is rescued at the early neurula stage by BMP blockade in β-catenin– but not VegT– embryos. Wild-type (uninjected, U), VegT– thioate (V), m β-catenin– thioate (T) and m/z β-catenin– morpholino (M) embryos, and siblings injected with cm-BMP7 (cmB) at the 8-cell stage (600 pg; 1 ventral vegetal cell) were frozen at the early gastrula (stage 10.5) and early neurula stage (stage 13) and analysed by real-time RT-PCR for organizer gene expression. The results are displayed as histograms to show the relative levels of expression. In each case, ornithine decarboxylase (ODC) is used as a loading control (data not shown), and each bar is normalized to the level of ODC expression.

Image published in: Xanthos JB et al. (2002)

Copyright © 2002. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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