Xenbase Image ID: 84428

Fig. S5. Specificity of Trio MO. Embryos were injected at the two cell stage as indicated, fixed at stage 25 and migration of neural crest cells was analysed by in situ hybridization against Xtwist. (A, B) Injection of TrioMO leads to a clear inhibition of neural crest migration at the injected side. (C, D) This inhibition of neural crest migration was rescued by co-injection of human Trio that is not recognized by Trio MO. (E) Quantification of inhibition in neural crest migration produced by TrioMO and its rescue by co-injection of human Trio DNA. This efficient rescue of TrioMO by Trio DNA shows the specificity of Xenopus TrioMO used in this work. Image published in: Moore R et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue twist1.S twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 25 neural crest cranial neural crest mandibular crest hyoid crest Image source: Published Larger Image Printer Friendly View

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