Xenbase Image ID: 84470

Fig. 3. Knockdown of endogenous Smurf1 results in neural defects. (A–D) Smurf1 MO (B) and Smurf1CA (D), but not control MO (A) or control β-gal mRNA (C) cause neural tube closure defects at neurula stages (top row) and microcephaly and microphtalmy at tailbud stages (bottom row). This phenotype is mimicked by 1–6 ng Smad1 mRNA overexpression (E). (F–I) F-actin accumulation at the neural fold hinge points at stage 18, revealed by phalloidin staining. Control embryos (F, H) have normal loop-shaped pattern. In Smurf1MO (G) or Smurf1CA embryos (I), f-actin staining is weak and the distance between the lateral hinge points is increased. Scale bar = 0.1 mm. Image published in: Alexandrova EM and Thomsen GH (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page