XB-IMG-84485
Xenbase Image ID: 84485
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Fig. 4. A-L: Developmental expression profile of Xenopus TK genes.
RNase protection analysis of Xenopus TK genes was performed using 10
p,g of total RNA (2.2 embryo equivalents) from oocytes (VI), unfertilized
eggs (E), and embryos of blastula (st. 5, 8, 9), gastrula (st. 10.5, 12),
neurula (st. 15, 19), tailbud (st. 21, 24), and hatching stage (st. 34). The
expression of ornithine decarboxylase (ODC), and EF-la are included as
controls. ["PI Labeled anti-sense probes were synthesized from the partial
TK cDNAs. The undigested probe and a background control (hybridization
with yeast tRNA) were included in each RNase protection analysis
experiment (not shown). Protected fragments were quantitated using a
Phosphorlmager (Molecular Dynamics), and the number of TK mRNA
molecules per embryo was estimated as described in Experimental Procedures.
The y-axis represents the TK mRNA levels displayed as units of
106 mRNA molecules per embryo. Image published in: Brändli AW and Kirschner MW (1995) Copyright © 1995. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |