Xenbase Image ID: 84529

Figure 4. Rescue of neural crest establishment defects, induced upon xPkp3-catenin depletion, through ectopic expression of xETV1.(A) The indicated morpholinos (20 ng) and mRNAs (0.5 ng) were co-injected into one dorsal blastomere at the four-cell stage with rhodamine dextran as a lineage tracer. After in situ hybridization for neural crest markers, Twist and FoxD3, embryos were imaged. The ectopic expression of ETV1-Myc largely rescued Twist and FoxD3 expression in embryos depleted for Pkp3-catenin (compare right-most images to middle images). (B) Quantification of embryos processed for Twist in situ hybrization, comparing the uninjected side to the injected side, indicated by an asterisk, for reduction in signal. (C) Quantification of embryos processed for FoxD3 in situ hybrization, comparing the uninjected side to the injected side for reduction in signal. P-values indicate statistical significance. Image published in: Munoz WA et al. (2014) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue twist1.S twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 15 neural crest cranial neural crest foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 15 neural crest cranial neural crest Image source: Published Larger Image Printer Friendly View

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