Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-IMG-84548

Xenbase Image ID: 84548


Fig. 1. The optimal dose of Cas9 mRNA for X. tropicalis embryos is 300 pg/embryo and the quantity of gRNA should not exceed 500 pg/embryo. (A) Representative morphology of dead and abnormal embryos evaluated when control siblings reached stage 30. The deformation was mainly caused by gastrulation defects. (B) Cas9 mRNA at 500 pg/embryo appeared toxic to X. tropicalis embryos. Dead and abnormal embryos were scored upon injection of Cas9 mRNA (500 pg/embryo) and gRNA (50 pg/embryo) targeting the indicated genes. (C) Constant amount (50 pg/embryo) of gRNA targeting hhex and graded doses of Cas9 mRNA (in pg/embryo) were injected into one-cell stage X. tropicalis embryos and the resulting dead and abnormal embryos were scored. (D,E) Constant amount of Cas9 mRNA (300 pg/embryo) and various doses (in pg/embryo) of gRNAs targeting either hhex (D) or pat (E) were injected into one-cell stage X. tropicalis embryos and the resulting dead and abnormal embryos were scored. (B-E) The total embryos for each injection (n) is given under each column.

Image published in: Guo X et al. (2014)

Copyright © 2014. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

Larger Image
Printer Friendly View

Return to previous page

My Xenbase: [ Log-in / Register ]
version: [4.5.0]

Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556