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Fig. 1. The optimal dose of Cas9 mRNA for X. tropicalis embryos is 300 pg/embryo and the quantity of gRNA should not exceed 500 pg/embryo. (A) Representative morphology of dead and abnormal embryos evaluated when control siblings reached stage 30. The deformation was mainly caused by gastrulation defects. (B) Cas9 mRNA at 500 pg/embryo appeared toxic to X. tropicalis embryos. Dead and abnormal embryos were scored upon injection of Cas9 mRNA (500 pg/embryo) and gRNA (50 pg/embryo) targeting the indicated genes. (C) Constant amount (50 pg/embryo) of gRNA targeting hhex and graded doses of Cas9 mRNA (in pg/embryo) were injected into one-cell stage X. tropicalis embryos and the resulting dead and abnormal embryos were scored. (D,E) Constant amount of Cas9 mRNA (300 pg/embryo) and various doses (in pg/embryo) of gRNAs targeting either hhex (D) or pat (E) were injected into one-cell stage X. tropicalis embryos and the resulting dead and abnormal embryos were scored. (B-E) The total embryos for each injection (n) is given under each column.
Image published in: Guo X et al. (2014)
Copyright © 2014. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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