Xenbase Image ID: 84549

Fig. 2. gRNA/Cas9 is an efficient and robust tool for gene targeting in X. tropicalis. (A) gRNA/Cas9 induced efficient targeted gene disruption in X. tropicalis embryos. The genes targeted and the doses of gRNAs (in pg/embryo) used are indicated. The dose of Cas9 mRNA was set at 300 pg/embryo for all injections in this figure. (B-G) Targeting efficiencies can be improved by increasing the amount of gRNA (pg/embryo shown), as evaluated with a constant amount of Cas9 mRNA (300 pg/embryo). (H) The targeting efficiencies of further gRNAs (50 pg/embryo). (I) At 15 pg/embryo, elastase-T1 gRNA was still 83.3% efficient. The numbers above each bar indicate mutations detected among total samples sequenced. Image published in: Guo X et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page