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XB-IMG-84549

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Fig. 2. gRNA/Cas9 is an efficient and robust tool for gene targeting in X. tropicalis. (A) gRNA/Cas9 induced efficient targeted gene disruption in X. tropicalis embryos. The genes targeted and the doses of gRNAs (in pg/embryo) used are indicated. The dose of Cas9 mRNA was set at 300 pg/embryo for all injections in this figure. (B-G) Targeting efficiencies can be improved by increasing the amount of gRNA (pg/embryo shown), as evaluated with a constant amount of Cas9 mRNA (300 pg/embryo). (H) The targeting efficiencies of further gRNAs (50 pg/embryo). (I) At 15 pg/embryo, elastase-T1 gRNA was still 83.3% efficient. The numbers above each bar indicate mutations detected among total samples sequenced.

Image published in: Guo X et al. (2014)

Copyright © 2014. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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