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Xenbase Image ID: 84564

Fig. 8. Whole-mount in situ hybridization analysis of G50 expression in neurula and early tailbud stage Xenopus embryos. A: Lateral view of a stage 17 embryo stained for G50 mRNA expression. Transcripts are detected in two bilateral stripes of cells in the head region (arrowheads), and diffusely in the posterior end of the embryo. 6: Dorsal view of A at higher magnification showing in greater detail the localization of G50 mRNAs in premigratory (arrow) and early migratory neural crest cell populations (arrowhead). C: Lateral view of a stage 21 embryo stained for Xenopus En-2 to localize the midbrain-hindbrain junction. D: Lateral view of a stage 21 embryo stained for G50. Expression of G50 mRNAs is seen in a stripe of cells at the height of the presumptive rhombomere 3, and in neural crest of the hyoid arch (h). E: Lateral view of a stage 21 embryo stained for Xenopus Krox-20. Two stripes of cells in the hindbrain corresponding to the presumptive rhombomeres 3 and 5, and neural crest of anterior branchial arch (ab) are expressing XKroxPO mRNAs. F Lateral view of a stage 21 embryo stained simultaneously for G50 and XKroxPO. Labeling of stained tissues is as in D and E, respectively. The intensity of the background stain varies with respect to the length of incubation of the embryos in the chromogenic reaction. Embryos in C and E were developed for 1 hr, while the other embryos were incubated 16 hr to obtain optimal staining. The stars in C and F indicate staining artifacts. Scale bars: A = 160 pm; 0 = 80 pm; C F = 144 pm.

Image published in: Brändli AW and Kirschner MW (1995)

Copyright © 1995. Image reproduced with permission of the Publisher, John Wiley & Sons.

egr2.LXKr20, EGR-2, Krox-20, XKrox-20, krox20X.laevisThroughout NF stage 21rhombomere R3
rhombomere R5
anterior branchial crest
en2.Lengrailed homolog 2, En-2, engrailed-2, engrailed 2, eng2, xen2, maben, en2-a, en2-bX.laevisThroughout NF stage 21presumptive midbrain-hindbrain boundary
epha2.LXE10X.laevisThroughout NF stage 17hindbrain
chordal neural plate
migratory neural crest cell
premigratory neural crest cell
epha2.LXE10X.laevisThroughout NF stage 21 to NF stage 56rhombomere R3
hyoid crest
tail region
neural tube

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