Xenbase Image ID: 84620

Fig. 4. Meta-I arrest by premature, ectopic expression of Erp1. (A) Inhibition of cyclin B2 degradation and Cdc2 inactivation after GVBD by premature, ectopic expression of Erp1. Immature oocytes were left uninjected (None) or injected with 1.2 ng of either Myc3-Erp1WT (WT) or Myc3-Erp1C583A (C583A) mRNAs, cultured overnight, treated with progesterone and processed as in Fig. 3E. Under these experimental conditions, both Erp1WT and Erp1C583A proteins were produced at endogenous Meta-II levels in oocytes undergoing GVBD (or MI) due to the leaky translation of the injected mRNA (see Fig. 2). (B) Spindle morphology in oocytes expressing ectopic Erp1. Oocytes expressing either Myc3-Erp1WT or Myc3-Erp1C583A as in panel A were subjected to immunostaining and DNA staining as in Fig. 3C. Note that Meta-I chromosomal configurations are maintained even long after GVBD only in oocytes expressing Erp1WT. The arrowhead shows a first polar body. Scale bar, 10 μm. Image published in: Ohe M et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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