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XB-IMG-84640

Xenbase Image ID: 84640


Figure 5. Depletion of VEGFA or Its Receptor, Flk1, Phenocopies the Tel1 Hemangioblast Phenotype (A) Injection of VegfA MO indicates that Gata2 and Lmo2 expression in the DLP is independent of VEGFA signaling, while Scl expression is dependent (arrows). Although a decrease in Gata2 expression is observed in the ventral blood island, VEGFA depletion appears to have little effect on developing embryonic erythrocytes (arrowheads). (B) Expression analysis in VegfA morphants showing that VEGFA is required for the expression of Flt1 and Flt4, but not Flk1, in the DLP (arrows). (C) Expression analysis in VegfA morphants showing that VEGFA is required for the formation of the DA (arrows). DA precursors located in the DLP do not migrate to the midline when VegfA is depleted. (D) Expression analysis in Flk1 morphants showing that Flk1 is required for SCL expression in the DLP, but that expression of Gata2 and Lmo2 is independent (arrows). (E) Expression analysis in Flk1 morphants showing that VEGFA is required for the expression of Flt1 and Flt4, but not Flk1 in the DLP (arrows). (F) Flk1 is required for the formation of the DA (arrows). DA precursors located in the DLP do not migrate to the midline when Flk1 is depleted. All embryos were hybridized as whole mounts and are shown in lateral view, with anterior to the left and dorsal to the top. Stages of development are as indicated. The numbers of embryos represented by each panel, out of the number analyzed, are indicated in the top right corner. See also Table S1.

Image published in: Ciau-Uitz A et al. (2010)

Copyright © 2010. Image reproduced on Xenbase with permission of the Publisher, Elsevier B. V.

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