XB-IMG-84643
Xenbase Image ID: 84643
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Figure 1. ETO2 Is Necessary for the Onset of Definitive Hematopoiesis(A) Expression analysis showing no difference in expression of primitive erythroid gene alpha T4 globin at stages 22 and 30 in WT and Eto2 morphant embryos. Blue arrowheads, VBI.(B and C) ETO2 morphants show absence of expression of Runx1, Gfi1, SpiB, and Scl in the DA (red arrows) at stage 39. (B) Whole-mount in situ hybridization, WMISH. White arrowheads indicate the notochord. The black arrow (Scl panel, WT embryo) indicates the posterior cardinal vein (PCV) containing Scl-positive primitive erythrocytes. Staining is absent in the ETO2 morphants because circulation is delayed. (C) In situ hybridization on section (ISHS) for SpiB and the endothelial marker Tie2; note that the DA has formed and is lumenized in Eto2 morphants. NT, neural tube. Numbers in the bottom of the panels denote the number of embryos as shown in the panel out of the total examined. Whole mounts are shown with anterior to the left and dorsal to the top. Sections are in transverse orientation with dorsal to the top. See also Figures S1 and S4. Image published in: Leung A et al. (2013) © 2013 ELL & Excerpta Medica. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |