XB-IMG-84655
Xenbase Image ID: 84655
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Figure S6.B. Vegfa hypomorph embryos and Vegfa-MOi6e7 morphants phenocopy the Eto2 morphants, related to Figure 6.
(B) Vegfa medium/long isoform morphants (Vegfa-MOi6e7) recapitulate the Eto2 morphant phenotype in the DA. WMISH analysis of hematopoietic (Gfi1, Gata2) endothelial (Flk1, CD31), arterial (Cx37 and Notch4) and hemangioblast (Scl, Flk1) markers reveals that endothelialisation, arterialisation and hematopoietic specification do take place in the morphant DA (red arrowheads). Note, however, the absence of ISVs (AA4, uncleared embryos, brackets), the weak staining of the PCVs (black arrows) and the limited development of the trunk vasculature (yellow arrows) in the Vegfa-MOi6e7 morphants. Numbers at the bottom of the panels indicate the number of embryos with the given phenotype out of the total number examined. Whole mounts are shown with anterior to the left and dorsal to the top.
Red arrowheads, DA; black arrows, PCV; brackets, ISVs; pink arrowheads, DLP; yellow arrowheads, VBI; dark blue arrowheads, Scl expression in the neural tube; green arrowheads, Flk1 expression in the trunk endothelium; light blue arrowheads, notochord; yellow arrows, trunk vasculature. Numbers at the bottom of the panels indicate the number of embryos with the given phenotype out of the total number examined. Whole mounts are shown with anterior to the left and dorsal to the top. Image published in: Leung A et al. (2013) © 2013 ELL & Excerpta Medica. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |