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Figure 3. Ectopic mitochondrial outer membrane (MOM) co-relocalization in HeLa cells of xETV1 with xPkp3, to map Pkp3's binding domains. (A) Co-expression of HA-xPkp3 deletion constructs with MOM-targeted Myc-xETV1, or co-expression of Myc-xETV1 with MOM-targeted HA-xPkp3. Cells were co-immunostained for the ectopic co-relocalization of xETV1 (Myc-epitope, left panels), as well as for xPkp3 (HA-epitope, middle panels). Ectopic co-relocalization of xPkp3 with MOM-targeted xETV1 was observed when amino acids 1-325 of xPkp3 were present, while co-relocalization of xPkp3's ARM domain was inconclusive due to its broad expression. Shown are representative images from a minimum of sixty cells that expressed both constructs and were analyzed. (B) List of HA-tagged (HA) xPkp3 constructs, and their interaction status with MOM-targeted Myc-xETV1 (or the inverse). A positive score (+) indicates that >60% of cells coexpressing the indicated xPkp3 deletion construct showed its co-relocalization with MOM-targeted Myc-xETV1. (C) Quantification of cells showing overlapping relocalization of MOM-targeted xPkp3 or xARVCF and xETV1 mapping constructs from Fig. 3A. Minimally 30 cells from each replicate experiment (totaling over 100 cells from triplicate experiments) were quantitated for their ability to co-relocalize. Image published in: Munoz WA et al. (2014) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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