Xenbase Image ID: 84737

Figure 1. Rasip1 Is Essential for Vascular Tubulogenesis in All Blood Vessels (A�D) Rasip1 expression is conserved in the embryonic vasculature across species shown by in situ hybridization: mouse (A, E9.5; B, E8.5 transverse section, left dorsal aorta), Xenopus tropicalis (C, st.30), and zebrafish (D, 24 hpf). (E�H) E9.5 littermate mouse embryos showing defects in Rasip1 / embryos (F) and yolk sacs (H). (I�L) Whole-mount Flk1-lacZ beta-galactosidase staining of Rasip1+/ (I and K) and Rasip1 / (J and L) yolk sacs (I and J) and embryonic heads (K and L), showing narrow Rasip1 / blood vessels that fail to remodel. (M�P) Connexin40 (Cx40 or Gja5) (M and N) or EphB4-lacZ (O and P) in littermate E9.5 Rasip1+/ and Rasip1 / yolk sacs (M and N) or embryos (O and P) showing failed arterial (M and N) and venous (O and P) differentiation in Rasip1 / vessels. (Q�Z0 ) Flk1-LacZ beta-galactosidase staining of littermate E8.5 (6 somite stage) Rasip1+/ and Rasip1 / embryos and tissues, in whole mount or section. (Q and R) Ventral views of E8.5 embryonic paired dorsal aortae. Anterior is up. (S�V) Transverse sections of E8.5 embryos, through the trunk region, posterior to the heart. (U and V) Close-up views, showing open lumens in Rasip1+/ ;Flk1-lacZ embryos (S and U) but lumenless cords in Rasip1 / ;Flk1-lacZ embryos (T and V). Sections through hearts of E8.5 (W and X), showing absence of a lumen in the endocardium of Rasip1 / ;Flk1-lacZ embryos (X). Yolk sacs in whole-mount (Y and Z) or section views (Y0 and Z0 ). Vascular tubes are absent in all Rasip1 / vessels examined. Arrows, dorsal aortae; Arrowheads, yolk sac vessels. Ys, yolk sac; Scale bars, 500 mm (A, E, F, G, and H), 250 mm (C), 200 mm (K�P), 100 mm (D, I, J, Q, R, Y, and Z), and 50 mm (B, S, T, U, V, W, X, Y, Z, Y0 , and Z0 ). See also Figures S1 and S2. Image published in: Xu K et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue rasip1.S X. laevis Throughout NF stage 26 blood vessel Image source: Published Larger Image Printer Friendly View

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