XB-IMG-84757
Xenbase Image ID: 84757
Fig. 7. Depletion of PouV proteins produces a heightened activin response in animal cap explants. (A) Real-time RT-PCR analysis of XlpouV protein-depleted animal cap explants. Embryos were injected with control MO or XlpouV MO as above. Animal cap explants were isolated at stage 8 and cultured with or without activin until sibling embryos reached stage 10.25. Relative expression represents values normalized to Odc. 16 U/ml activin was used for induction apart from the Xbra and Bmp4 analysis that used 8 U/ml activin. Experiments were carried out in triplicate. (B) Embryos were injected with control MO or XlpouV MO as above and animal caps explants isolated and induced with either 8 U/ml or 16 U/ml activin. Relative expression represents the Gsc and cerberus values normalized to Odc. Experiments were carried out in triplicate. Image published in: Morrison GM and Brickman JM (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |