XB-IMG-84863
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Figure 4. Slc gene expression defines segmentation of the intermediate, distal, and connecting tubules. Stage 35/36 Xenopus embryos were stained for marker gene expression by whole-mount in situ hybridization. Lateral views of stained embryos are shown accompanied by enlargements of the pronephric region and a color-coded scheme of the nephron depicting the deduced segmental expression domains. (a) slc19a2: intermediate tubule. (b) slc20a1: intermediate tubule (IT)1 (arrowheads). (c) slc5a8: proximal tubule (PT)2, PT3, IT2, and distal tubule (DT)1. In the upper panel, the embryo was stained to reveal slc5a8 expression in IT2 (arrow) and DT1 (arrowhead). The embryo shown in the lower panel was stained shorter to demonstrate expression in PT2 (arrowhead) and PT3 (arrows). (d) slc4a4: proximal tubules, DT1. Arrowheads illustrate expression in PT1. (e) slc12a1: intermediate tubule, DT1. (f) rhcg/slc42a3: DT2. (g) slc8a1: connecting tubule (CT). (h) slc12a3: DT2, CT. Note that there is also strong slc12a3 expression in the cloaca (arrowhead). The localization of the expression domains for slc12a1 and slc12a3 has previously been reported [22]. They are shown here for comparative purposes. Image published in: Raciti D et al. (2008) Copyright © 2008 Raciti et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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