Xenbase Image ID: 84864

Figure 5. Expression domains of selected molecular marker genes validates the pronephric segmentation model. Whole-mount in situ hybridizations of stage 35/36 Xenopus embryos were performed. Lateral views of whole embryos (left panels), enlargements of the pronephric region (middle panels), and color-coded schematic representations of the segment-restricted expression domains (right panels) are shown. (a) cldn8: intermediate tubule (IT)2. Note that the expression levels are low. Arrowheads indicate the proximal and distal boundaries of the cldn8 expression domain. (b,d) cldn14 and cldn16: intermediate tubule, distal tubule (DT)1. (d) cldn19: nephrostomes (arrowheads), intermediate tubule. (e) clcnk: intermediate tubule, distal tubule, connecting tubule. Note that the dotted staining pattern localizes to cells of the epidermis. The localization of the clcnk expression domains has previously been reported [22] and is shown here for comparative purposes. (f) kcnj1: IT1, distal tubule, connecting tubule. The arrowhead indicates the location of IT2, which fails to express kcnj1. (g) calb1: connecting tubule. The arrowhead indicates the proximal boundary of the expression domain. Expression is highest in the most distal parts of the connecting tubule. Image published in: Raciti D et al. (2008) Copyright © 2008 Raciti et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue cldn14.L claudin-14, DFNB29 X. laevis Throughout NF stage 35 and 36 distal tubule pronephric kidney early distal tubule late distal tubule head cldn8.4 claudin-8 X. tropicalis Throughout NF stage 35 and 36 distal tubule pronephric kidney cldn16.L claudin-16, LOC108718049, PCLN1 X. laevis Throughout NF stage 35 and 36 distal tubule pronephric kidney early distal tubule late distal tubule clcnkb.L ClC-k, clc-kb, clckb, clcnka, clk-k2, xClC-k X. laevis Throughout NF stage 35 and 36 distal tubule pronephric kidney pronephric duct early distal tubule late distal tubule epidermis kcnj1.L kir1.1, MGC68935, ROMK, romk1 X. laevis Throughout NF stage 35 and 36 distal tubule pronephric kidney pronephric duct early distal tubule late distal tubule calb1.L calb, calb1-a, calb1-b, calbindin X. laevis Throughout NF stage 35 and 36 head brain eye pharyngeal region otic vesicle posterior pronephric duct retina Image source: Published Larger Image Printer Friendly View

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