Xenbase Image ID: 85049

Figure S7. The xNorrin K57N mutant failed to efficiently rescue anterior defects in xNorrin morphants. (A-E) An uninjected embryo (A). A xNor-MO (20 ng)-injected embryo (B). Note the lack of eye pigment. A xNor-misMO (20 ng)-injected embryo (C). A xNor-MO and wild-type Norrin RNA (50 pg) co-injected embryo (D). A xNor-MO and xNorrin K57N RNA (50 pg) co-injected embryo (E). (F) Summary of anterior defect frequency in (A-E). Uninjected: n = 40; MO: n = 24; xNor rescue: n = 20; K57N rescue: n = 19; misMO: n = 15. RNAs and MO were injected into the dorsal animal region at the four-cell stage. All embryos shown are around stage 36. Image published in: Xu S et al. (2012) Xu et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page