Xenbase Image ID: 85165

Fig. 5. Xlmx1b is expressed in the nervous system and glomus domains at the same time. RT-PCR analysis shows Xlmx1b expression is initiated ubiquitously at stage 10.5. Transcripts are then found in dorsal and lateral regions of stage 13 and 20 embryos, as these two regions will eventually form the nervous system and glomus tissues respectively. At stage 25 and 35, expression is seen in the head from the nervous system expression domain, in the mid region from both nervous system and glomus domains and in the tail region from the neurons. xWT-1 was used to identify the glomus domain and show that Xlmx1b expression in these regions is initiated prior to xWT-1. This experiment cannot rule out expression in those regions being due to some contamination with the neural domain. Dissection was as follows; W, whole embryos; D, dorsal; L, lateral; V, ventral; H, head; M, middle and T, tail regions. (cDNA was not equalised in this experiment in order to establish a genuine relative expression level between components). Image published in: Haldin CE et al. (2003) Copyright © 2003. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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