XB-IMG-85173
Xenbase Image ID: 85173
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Fig. 1. HMTase activity and developmental expression of Prdm13. (A) mPrdm13 is a histone methyl-transferase. Core histones were used as substrates in a HMTase assay with a immunopurified Flag-Prdm13 protein produced in 293T cells. Flag-Prdm16 was used as a positive control. Empty vector transfected cells were used to control for nonspecific background. The results represent the average of triplicate samples plus SD. The amount of immunoprecipitated Prdm16 and Prdm13 proteins used in the assay is shown on the western on the right (arrows). Note that mPrdm13 is expressed at a much lower level than mPrdm16. On the autoradiograph shown, multiple bands are seen for mPrdm16 due to the high exposure time used to detect mPrdm13. Those bands likely correspond to mPrdm16 degradation products. A non-specific IgG band is indicated by an arrowhead. (B) Temporal embryonic expression of Xenopus laevis Prdm13 as determined by RT-PCR. Histone H4 is shown as a loading control. (C–K) Whole-mount in situ analysis of Prdm13 expression during embryogenesis. Panel E, transversal section of the neural tube of a stage 22 embryo. Panels H and I, transversal sections of the neural tube of a stage 32 embryo at the levels indicated in G. In panel K, transversal section of the neural tube of an embryo that has been additionally stained for proliferation by EdU incorporation. The enlargement in the inset shows that some Prdm13 are EdU positive. Nieuwkoop Faber stages are indicated. All embryos shown are with anterior to the right. (C, D) Dorsal views. (F, G, J) Lateral views. Image published in: Hanotel J et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V.
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