Xenbase Image ID: 85175

Fig. 3. Prdm13 is dramatically affected by Ptf1a but not by Neurog2 gain or loss of function in Xenopus. (A–L) In situ hybridization analysis of Prdm13 expression in Ptf1a-GR, Ptf1a-VP16 or Neurog2-GR injected embryos analyzed at neurula and tadpole stages. Pax2 was used as a control. At neurula stage, embryos are shown in dorsal views, anterior to the bottom. At tadpole stage, transversal sections at the level of the posterior hindbrain or spinal cord are shown. The arrow in G indicates ectopic Prdm13 in the somites. (M–R) In situ hybridization analysis of Prdm13 expression in embryos injected with 20 ng of a Ptf1a-MO or a Neurog2-MO. Pax2 was used as a control for the Ptf1a-MO. Prdm13 expression was analyzed at the open neural plate stage and in the neural tube of tadpole stage embryos. Pax2 was only analyzed at tadpole stage. Note that Prdm13 like Pax2 is reduced in Ptf1a-MO injected embryos and is largely unaffected in Neurog2-MO injected embryos. In all cases, LacZ mRNA was used as a lineage tracer. IS, injected side. Image published in: Hanotel J et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + ptf1a-GR + DEX + neurula stage (in situ hybridization) A Expression Phenotype mislocalised prdm13.L expression in ectoderm increased amount prdm13.L expression in ectoderm increased amount prdm13.L expression in neural plate mislocalised prdm13.L expression in pre-chordal neural plate Xla Wt + ptf1a-GR + DEX + neurula stage (in situ hybridization) B Expression Phenotype increased amount pax2.L expression in presumptive midbrain-hindbrain boundary Xla Wt + ptf1a-GR + DEX + tadpole stage (in situ hybridization) C Expression Phenotype mislocalised prdm13.L expression in ectoderm increased amount prdm13.L expression in ectoderm Xla Wt + ptf1a-GR + DEX + tadpole stage (in situ hybridization) D Expression Phenotype mislocalised pax2.L expression in ectoderm increased amount pax2.L expression in ectoderm increased amount pax2.L expression in neural tube Xla Wt + ptf1a-VP16 + neurula stage (in situ hybridization) E Expression Phenotype increased amount prdm13.L expression in ectoderm mislocalised prdm13.L expression in ectoderm Xla Wt + ptf1a-VP16 + neurula stage (in situ hybridization) F Expression Phenotype increased amount pax2.L expression in neural fold increased amount pax2.L expression in presumptive midbrain-hindbrain boundary Xla Wt + ptf1a-VP16 + tadpole stage (in situ hybridization) G Expression Phenotype mislocalised prdm13.L expression in ectoderm increased amount prdm13.L expression in ectoderm increased amount prdm13.L expression in somite mislocalised prdm13.L expression in somite Xla Wt + ptf1a-VP16 + tadpole stage (in situ hybridization) H Expression Phenotype mislocalised pax2.L expression in ectoderm increased amount pax2.L expression in ectoderm Xla Wt + ptf1a MO + neurula stage (in situ hybridization) M Expression Phenotype decreased amount prdm13.L expression in neural fold Xla Wt + ptf1a MO + tadpole stage (in situ hybridization) N Expression Phenotype decreased amount prdm13.L expression in neural tube Xla Wt + ptf1a MO + tadpole stage (in situ hybridization) O Expression Phenotype decreased amount pax2.L expression in neural tube Xla Wt + neurog2 MO + neurula stage (in situ hybridization) P Expression Phenotype decreased amount prdm13.L expression in neural fold Xla Wt + neurog2 MO + tadpole stage (in situ hybridization) Q Expression Phenotype decreased amount prdm13.L expression in neural tube Larger Image Printer Friendly View

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