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Fig. 4. Prdm13 is a likely direct Ptf1a transcriptional target. (A) Real time RT-PCR analysis of Prdm13 expression in animal cap explants derived from Ptf1a-GR overexpressing embryos either left untreated or exposed to DEX for 1–6 h before harvesting. Pax2, was used as a control. Note that Prdm13 expression is already dramatically induced after 4 h of treatment of DEX. In contrast, Pax2 is not induced at those early time points of induction. CC, control caps. (B) In situ hybridization analysis of Prdm13 and Pax2 in neurula embryos overexpressing Ptf1a-GR, treated with or without CHX for 1 h at stage 12.5 before the addition (or not) of DEX as indicated. LacZ mRNA was used as a lineage tracer. Dorsal views are shown, with anterior to the bottom. IS, injected side. (C) Real time RT-PCR analysis of the expression of Prdm13 and Pax2 in animal caps derived from Ptf1a-GR overexpressing embryos, pretreated or not with CHX before the addition (or not) of DEX as in (B). Note that both in embryos and in animal caps, this CHX treatment does not reduce Prdm13 induction while Pax2 induction is completely abolished. Image published in: Hanotel J et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + ptf1a-GR + CHX + DEX + NF15-16 (in situ hybridization) B Expression Phenotype mislocalised prdm13.L expression in neural plate increased amount prdm13.L expression in neural plate Xla Wt + ptf1a-GR + DEX + NF15-16 (in situ hybridization) B Expression Phenotype increased amount pax2.L expression in neural fold mislocalised prdm13.L expression in ectoderm increased amount prdm13.L expression in ectoderm Larger Image Printer Friendly View

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