Xenbase Image ID: 85179

Fig. 7. Knockdown of Prdm13 reduces Pax2 and an upregulates Tlx3 in the dorsal neural tube of early tadpole embryos and in Ptf1a overexpressing animal caps. (A–H) Dorsal view and (I–L) transversal sections at the level of the posterior hindbrain or anterior spinal cord of Prdm13-MO injected embryos hybridized with the indicated probes. Note that Pax2 is markedly reduced in the dorsal neural tube (arrow) while its more ventral expression domain is unaffected. In contrast, Tlx3 and Prdm13 are upregulated. Sox3 is unperturbed by injection of the Prdm13-MO. Injection of a Prdm13-MOmis has no effect on the different markers tested. (M-R) Real-time RT-PCR analysis of animal cap explants isolated from embryos injected with Ptf1a mRNA and morpholinos as indicated and collected when sibling embryos reached stage 26. Note that the knockdown of Prdm13 inhibits the increase of Pax2 and Gad1 and upregulates Tlx3, Vglut1 and Lmx1b. Such an effect is not seen with a Prdm13-MOmis or a standard control MO (MOctrl). Prdm13 is also induced by Ptf1a. The activation of Prdm13 by Ptf1a (430 fold activation) is not seen on the graph due to the strong activation in the presence of the Prdm13-MO (19,000 fold activation). CC, control caps. Image published in: Hanotel J et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + prdm13 MO + tadpole stage (in situ hybridization) E, I Expression Phenotype increased amount pax2.L expression in dorsolateral neural tube Xla Wt + prdm13 MO + tadpole stage (in situ hybridization) F, J Expression Phenotype increased amount tlx3.S expression in neural tube Xla Wt + prdm13 MO + tadpole stage (in situ hybridization) H, L Expression Phenotype increased amount prdm13.L expression in neural tube Larger Image Printer Friendly View

Return to previous page