Xenbase Image ID: 85185

Fig. 4. Fmi can block the posteriorisation of neural tissue by Wnt3A. (A) Schematic overview of the experimental procedure. Fertilised Xenopus eggs were injected with various combinations of RNAs (1) and cultured until the early gastrula stage (2). At this point the animal cap was removed (3) and cultured until sibling embryos had reached the early tail bud stage. Total RNA was extracted and analysed for marker gene expression by RT-PCR. (B) RTPCR analysis of caps expressing a combination of different injected RNAs, as shown (�TREATMENT�). The expression of various marker genes was analysed by RT-PCR (�MARKER�). a/s MFmi, morpholino oligo with complementary sequence to the translation start site of the MFmi RNA. XOtx2, XHoxb1, XHoxb4 and XHoxb9 are arranged in anterior (Ant) to posterior (Post) order, as indicated by the double headed arrow. Image published in: Morgan R et al. (2003) Copyright © 2003. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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