Xenbase Image ID: 85190

Fig. S2. Prdm13 expression in the developing retina. (A, B, D-F) Retinal sections after whole mount in situ hybridization with the indicated probes. (A) Prdm13 transcripts start to be expressed from stage 28 onwards in a subpopulation of retinal progenitors. (B) From stage 33/34, Prdm13 is expressed in the inner nuclear layer. (C) In situ hybridization with Prdm13 on cryostat section and EdU labeling (green) showing that some Prdm13 positive cells in the CMZ are in proliferation. (D-F) At stage 42, Prdm13 is restricted to the inner part of this layer where amacrine cells reside (see the enlargement in E). Prdm13 is also expressed in the ciliary marginal zone (CMZ), excluding the most peripheral region, similarly to Ptf1a. (G-I) Images of stage 40 retinal section focusing on the ventral CMZ region, following double fluorescent in situ hybridization. Many cells co-express Ptf1a and Prdm13 (arrows). OP: optic vesicle, CMZ: ciliary marginal zone, GCL: ganglion cell layer, iINL: inner part of the inner nuclear layer, oINL: outer part of the inner nuclear layer, ONL: outer nuclear layer. Scale bars: 100μm (A-D, F), 50μm (G-I). Image published in: Hanotel J et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue prdm13.L X. laevis Throughout NF stage 28 retina prdm13.L X. laevis Throughout NF stage 33 and 34 retina interneuron retinal neural layer prdm13.L X. laevis Sometime during NF stage 40 to NF stage 42 retina amacrine cell interneuron retinal inner nuclear layer ciliary marginal zone ptf1a.S ptf1a-a, ptf1a-b, Ptf1a/p48, XPtf1a X. laevis Sometime during NF stage 40 to NF stage 42 retina ciliary marginal zone Image source: Published Larger Image Printer Friendly View

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